Project information
Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
- Project Identification
- MSM0021622430
- Project Period
- 1/2007 - 12/2013
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
- Faculty of Medicine
- Other MU Faculty/Unit
- Faculty of Science
- Other MU Faculty/Unit
- Faculty of Informatics
- Other MU Faculty/Unit
- Central European Institute of Technology
- Keywords
- embryonic stem cells
The goal of this project will be a) to profit from availability of unique cellular resources - mouse and human embryonic stem cells and normal and malignant hematopoietic stem/progenitor cells, b) to effectively employ a wide variety of current and complementary methodologies available to the consortium, and c) to exploit a proven/certified expertise in working with various living systems that span from in vitro cultured cells, through animal models to oncohematological patients. All this together will represent a powerful experimental platform aimed at addressing a nature of molecules and molecular mechanisms underlaying phenomena that are common to both normal and cancer stem cells, mainly their ability to self-renew and to enter differentiation pathways. Integral part of this general goal is to implement multifaceted analytical approach that will cover studies of gene expression at both mRNA and protein levels, evaluating function of extra and intracellular signaling pathways, and analyzing biological properties of cells in vitro and in vivo. Expected outcome will be not only understanding but also a definition of molecules and/or molecular pathways to be tested as therapeutic targets, and cellular models that will make such testing feasible.
Publications
Total number of publications: 361
2010
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Increasing hematopoietic microchimerism is a reliable indicator of incipient AML relapse
International Journal of Laboratory Hematology, year: 2010, volume: 33, edition: 1
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Inhibition of Notch Signaling in Human Embryonic Stem Cell-Derived Neural Stem Cells Delays G1/S Phase Transition and Accelerates Neuronal Differentiation In Vitro and In Vivo
Stem Cells, year: 2010, volume: 28, edition: 5, DOI
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Interactions with the microenvironment protect lymphoma B-cells from rituximab induced apoptosis and could represent a therapeutic target.
Year: 2010, type: Conference abstract
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Isolation of granulocytes: Which transcriptome do we analyze neutrophils or eosinophils?
FOLIA BIOLOGICA, year: 2010, volume: 56, edition: 6
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Laboratorní kazuistika dvou případů APL s atypickými zlomy fúzního genu PML/RARa
Year: 2010, type: Conference abstract
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Long-term cultivation of human embryonic stem cells (hESCs) - signs of an adaptation to in vitro culture conditions at 3 cell lines
Year: 2010, type: Conference abstract
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Manipulation of gene expression in chronic lymphocytic leukemia cells in vitro
Year: 2010, type: Conference abstract
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MicroRNA v patogenezi chronické lymfocytární leukemie a jejich prognostický význam
Transfuze a hematologie dnes, year: 2010, volume: 16, edition: Suppl. 1
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Minimal residual resistance in CML: Stable BCR-ABL gene expression at levels 0.01-0.1% (IS) in the consecutive samples may be associated with BCR-ABL mutation development and MMoR lost in a small number of tyrosine kinase inhibitor responders.
Year: 2010, type: Conference abstract
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Monitoring of minimal residual disease in acute myeloid leukemia with frequent and rare patient-specific NPM1 mutations
American Journal of Hematology, year: 2010, volume: 85, edition: 12