Project information
Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
- Project Identification
- MSM0021622430
- Project Period
- 1/2007 - 12/2013
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
- Faculty of Medicine
- Other MU Faculty/Unit
- Faculty of Science
- Other MU Faculty/Unit
- Faculty of Informatics
- Other MU Faculty/Unit
- Central European Institute of Technology
- Keywords
- embryonic stem cells
The goal of this project will be a) to profit from availability of unique cellular resources - mouse and human embryonic stem cells and normal and malignant hematopoietic stem/progenitor cells, b) to effectively employ a wide variety of current and complementary methodologies available to the consortium, and c) to exploit a proven/certified expertise in working with various living systems that span from in vitro cultured cells, through animal models to oncohematological patients. All this together will represent a powerful experimental platform aimed at addressing a nature of molecules and molecular mechanisms underlaying phenomena that are common to both normal and cancer stem cells, mainly their ability to self-renew and to enter differentiation pathways. Integral part of this general goal is to implement multifaceted analytical approach that will cover studies of gene expression at both mRNA and protein levels, evaluating function of extra and intracellular signaling pathways, and analyzing biological properties of cells in vitro and in vivo. Expected outcome will be not only understanding but also a definition of molecules and/or molecular pathways to be tested as therapeutic targets, and cellular models that will make such testing feasible.
Publications
Total number of publications: 359
2014
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Interaction of Notch and gp130 Signaling in the Maintenance of Neural Stem and Progenitor Cells
Cellular and Molecular Neurobiology, year: 2014, volume: 34, edition: 1, DOI
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MALDI-TOF fingerprinting of the whole mammalian cell-lines
Year: 2014, type: Appeared in Conference without Proceedings
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Molecular and Functional Characterization of Uniform-Sized Beating Embryoid Bodies and Cardiomyocytes from Human Embryonic and Induced Pluripotent Stem Cells
Year: 2014, type: Conference abstract
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Mutation frequency dynamics in HPRT locus in culture adapted hESCs and iPSCs correspond to their differentiated counterparts
Stem Cells and Development, year: 2014, volume: 23, edition: 20, DOI
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NOD/SCID gamma xenograft model of B-cell malignancies using TP53- and/or ATM-deficient cell lines
Year: 2014, type: Conference abstract
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Optimization of cardiomyocytes differentiation from pluripotent stem cells using atomic force microscopy
Year: 2014, type:
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Phenotype-genotype correlations in patients with Marinesco-Sjogren syndrome
Clinical Genetics, year: 2014, volume: 86, edition: 1, DOI
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Tissue profiling by nanogold-mediated mass spectrometry and artificial neural networks in the mouse model of human primary hyperoxaluria 1
Journal of Applied Biomedicine, year: 2014, volume: 12, edition: 2, DOI
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Tissue visualization mediated by nanoparticles: from tissue staining to mass spectrometry tissue profiling and imaging
Nanomedicine, edition: Vyd. 1st, year: 2014, number of pages: 22 s.
2013
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Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability
STEM CELLS Translational Medicine, year: 2013, volume: 2, edition: 4, DOI