Project information
Comprehensive prognostic and predictive panel for chronic lymphocytic leukemia: a next-generation sequencing tool suitable for clinical practice and study of genetic architecture behind the disease progress
- Project Identification
- NV19-03-00091
- Project Period
- 5/2019 - 12/2022
- Investor / Pogramme / Project type
-
Ministry of Health of the CR
- Ministry of Health Research Programme 2015 - 2022
- MU Faculty or unit
- Central European Institute of Technology
- Cooperating Organization
-
University Hospital Brno-Bohunice
- Responsible person Mgr. Veronika Navrkalová, Ph.D.
Chronic lymphocytic leukemia (CLL) is common and still incurable leukemia of adults characterized by remarkable genetic and clinical heterogeneity. In proposed project we aim to study genetic background underlying clonal evolution of genomic alterations in treatment naïve CLL patients taking the advantage of availability of consecutive samples collected during disease course. Our main objective is to early disclose the disease progression and therapy need. We plan to assess both the prognostic and the predictive value of genomic aberrations detected just before treatment start. For this purpose, a highly complex next-generation sequencing (NGS) tool analyzing simultaneously both fundamental, as well as novel CLL biomarkers will be implemented. We employ benefits of capture-based NGS technology enabling to analyze in one test IGHV mutational status, recurrent chromosomal and gene defects, and Ig light chains clonality. We presume that our NGS panel tailored for CLL can be widely adopted in clinical practice as a simple comprehensive test for better patient prognostication.
Publications
Total number of publications: 47
2022
-
Impact of the Types and Relative Quantities of IGHV Gene Mutations in Predicting Prognosis of Patients With Chronic Lymphocytic Leukemia
Frontiers in Oncology, year: 2022, volume: 12, edition: July 2022, DOI
-
In-frame deletions and insertions in the TP53 gene identified in leukemia patients result in p53 protein inactivation.
Year: 2022, type: Conference abstract
-
Integrated NGS testing and cfDNA analysis as an approach for aggregate tumor characterization and monitoring in a patient with aggressive lymphoma invading the central neural system.
Year: 2022, type: Conference abstract
-
Komplexní analýza nádorového genomu pacientů s mnohočetným myelomem.
Year: 2022, type: Conference abstract
-
Komplexní cytogenomická a molekulárně biologická analýza nádorového genomu pacientů s myeloidními malignitami se zaměřením na cílenou analýzu genu MECOM.
Year: 2022, type: Conference abstract
-
Loss of heterozygosity in EIF3F gene leading to intellectual disability in two siblings.
Year: 2022, type: Conference abstract
-
LYNX: A web-based bioinformatic tool for targeted next-generation sequencing data analysis and visualization in lymphoid malignancies.
Year: 2022, type: Conference abstract
-
Pathogenic germline variants of TP53 gene are rare in patients with chronic lymphocytic leukemia.
Year: 2022, type: Conference abstract
-
Realita zapojení molekulárně biologických metod do diagnostiky a stratifikace lymfoproliferativních onemocnění.
Year: 2022, type: Conference abstract
-
Současná role cytogenomiky v diagnostice a prognostické stratifikaci hematologických malignit.
Year: 2022, type: Conference abstract