Project information
Mechanism of procathepsin D effect on breast cancer cells

Project Identification

IAA501630801

Project Period

1/2008 - 12/2011

Investor / Pogramme / Project type

Academy of Sciences of the Czech Republic

Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR

MU Faculty or unit

Faculty of Science

Keywords

procathepsin D, breast cancer, protein interactions, apoptosis, cell signaling

Procathepsin D (pCD) is over-expressed and secreted by several types of tumors and cancer cell lines. Increased level of pCD is known to affect multiple stages of tumor progression, including proliferation, invasion and metastasis and is associated with poor prognosis of various cancers. It has been suggested that protein-protein interactions are important mediators of these effects. Using gene microarray approach we have recently identified several differentially expressed genes upon treatment of breast cancer cells with pCD. This project is therefore designed to A) explore the role of NF-kB2 and MAPK in mediating the effects of pCD on breast cancer cells, B) search for pCD protein-protein interacting partners, C) analyze the role of pCD-mediated signaling in regulation of apoptosis of breast cancer cells. We believe that this project can answer the question how the pCD affects cancer cells. Full understanding of this mechanism is important for design of pCD-targeted cancer therapy.

Publications

Total number of publications: 20

2012

Redox State Alters Anti-Cancer Effects of Wedelolactone

BENEŠ Petr ALEXOVA Petra KNOPFOVÁ Lucia ŠPANOVÁ Alena ŠMARDA Jan

Article in Periodical

Environmental and Molecular Mutagenesis, year: 2012, volume: 53, edition: 7, DOI

2011

Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity

BENEŠ Petr JANČEKOVÁ Blanka KNOPFOVÁ Lucia ŠMARDA Jan

Conference abstract

Year: 2011, type: Conference abstract
c-Myb regulates MMP1, MMP9 and cathepsin D: Implications for the matrix-dependent breast cancer cell invasion and metastasis

KNOPFOVÁ Lucia BENEŠ Petr PEKARČÍKOVÁ Lucie HERMANOVÁ Markéta MASAŘÍK Michal SOUČEK Karel ŠMARDA Jan

Conference abstract

Year: 2011, type: Conference abstract
c-Myb-induced migration/invasiveness of breast and colon cells in vitro is not associated with enhanced lung colonization in vivo

KNOPFOVÁ Lucia PEKARČÍKOVÁ Lucie BENEŠ Petr PERNICOVÁ Zuzana SOUČEK Karel MASAŘÍK Michal ŠMARDA Jan

Conference abstract

Year: 2011, type: Conference abstract
Inhibition of topoisomerase IIa: Novel function of wedelolactone

BENEŠ Petr KNOPFOVÁ Lucia TRČKA Filip NEMAJEROVÁ Alice PINHEIRO Diana SOUČEK Karel FOJTA Miroslav ŠMARDA Jan

Article in Periodical

Cancer Letters, year: 2011, volume: 303, edition: 1, DOI
OVEREXPRESSION OF CATHEPSIN D SENZITIZES BREAST CANCER CELLS TO TRAIL-INDUCED APOPTOSIS

JANČEKOVÁ Blanka BENEŠ Petr ONDROUŠKOVÁ Eva ŠMARDA Jan

Conference abstract

Year: 2011, type: Conference abstract
The effects of wedelolactone on cancer cells depend on its redox state

BENEŠ Petr ALEXOVA Petra KNOPFOVÁ Lucia ŠMARDA Jan

Conference abstract

Year: 2011, type: Conference abstract
The Overexpression of Cathepsin D Senzitizes Brest Cancer Cells to TRAIL-induced Apoptosis

JANČEKOVÁ Blanka BENEŠ Petr ONDROUŠKOVÁ Eva ŠMARDA Jan

Conference abstract

Year: 2011, type: Conference abstract

2010

c-Myb promotes invasivity of breast cancer cells

KNOPFOVÁ Lucia TRČKA Filip BENEŠ Petr ŠMARDA Jan

Conference abstract

Year: 2010, type: Conference abstract
c-Myb promotes motility and invasion of breast cancer cells

KNOPFOVÁ Lucia TRČKA Filip BENEŠ Petr ŠMARDA Jan

Conference abstract

Year: 2010, type: Conference abstract