Project information
Delta podjednotka RNA polymerázy z Gram pozitivních bakterií (DELTA)

Information

This project doesn't include Faculty of Science. It includes Central European Institute of Technology. Official project website can be found on muni.cz.

Project Identification

GA204/09/0583

Project Period

1/2009 - 12/2012

Investor / Pogramme / Project type

Czech Science Foundation

Standard Projects

MU Faculty or unit

Central European Institute of Technology

prof. Mgr. Lukáš Žídek, Ph.D.

Keywords

RNA polymerase

Cooperating Organization

Institute of Microbiology of the ASCR, v. v. i.

Responsible person Mgr. Libor Krásný, Ph.D.

To efficiently fight bacterial diseases and infections, knowledge of the molecular mechanisms used by these organisms is required. Bacterial RNA polymerase (RNAP) is an essential multisubunit enzyme. Contrary to the well studied RNAPs from gram negative bacteria, gram positive RNAPs contain the d subunit. Its role in the cell is not well understood. We propose to conduct structural, genetic, and biochemical studies to define its role in gene expression regulation. We propose to solve the 3D structure of delta, determine its position within RNAP, and use this new knowledge to define its physiological role in the cell.

Publications

Total number of publications: 19

2010

NMR as a tool for studies of (partially) disordered proteins and their interactions

PAPOUŠKOVÁ Veronika ŽÍDEK Lukáš SKLENÁŘ Vladimír KRÁSNÝ Libor KOZMINSKI Wiktor

Conference abstract

Year: 2010, type: Conference abstract
S3EPY: a Sparky extension for determination of small scalar couplings from spin-state-selective excitation NMR experiments

NOVÁK Petr ŽÍDEK Lukáš MOTÁČKOVÁ Veronika PADRTA Petr ŠVENKOVÁ Alžbeta NUZILLARD Jean Marc KRÁSNÝ Libor SKLENÁŘ Vladimír

Article in Periodical

Journal of Biomolecular NMR, year: 2010, volume: 46, edition: 2
Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs

MOTÁČKOVÁ Veronika ŠANDEROVÁ Hana ŽÍDEK Lukáš NOVÁČEK Jiří PADRTA Petr ŠVENKOVÁ Alžběta KORELUSOVÁ Jana JONÁK Jiří KRÁSNÝ Libor SKLENÁŘ Vladimír

Article in Periodical

Proteins: Structure, Function, and Bioinformatics, year: 2010, volume: 78, edition: 7
Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments

MOTÁČKOVÁ Veronika NOVÁČEK Jiří ZAWADZKA-KAZIMIERCZUK Anna KAZIMIERCZUK Krzysztof ŽÍDEK Lukáš ŠANDEROVÁ Hana KRÁSNÝ Libor KOZMINSKI Wiktor SKLENÁŘ Vladimír

Article in Periodical

Journal of Biomolecular NMR, year: 2010, volume: 48, edition: 3
Structure and dynamics of an RNA polymerase subunit unique for gram-positive bacteria

MOTÁČKOVÁ Veronika KADEŘÁVEK Pavel ŽÍDEK Lukáš ŠANDEROVÁ Hana PADRTA Petr NOVÁČEK Jiří ŠVENKOVÁ Alžběta KORELUSOVÁ Jana ZAWADZKA-KAZIMIERCZUK Anna KAZIMIERCZUK Krzysztof KOŹMIŃSKI Wiktor KRÁSNÝ Libor SKLENÁŘ Vladimír

Conference abstract

Year: 2010, type: Conference abstract
Structure and dynamics of RNA polymerase delta subunit from Bacillus subtilis determined by NMR spectroscopy

MOTÁČKOVÁ Veronika

Conference abstract

Year: 2010, type: Conference abstract
Structure determination of an RNA polymerase subunit unique for gram-positive bacteria

MOTÁČKOVÁ Veronika ŽÍDEK Lukáš SKLENÁŘ Vladimír

Conference abstract

Year: 2010, type: Conference abstract

2009

NMR structural study of N-terminal domain of RNA polymerase delta subunit from Bacillus subtilis

MOTÁČKOVÁ Veronika ŠANDEROVÁ Hana NOVÁČEK Jiří ŽÍDEK Lukáš KRÁSNÝ Libor SKLENÁŘ Vladimír

Conference abstract

Year: 2009, type: Conference abstract
Structural study of an RNA polymerase subunit unique for gram-positive bacteria

MOTÁČKOVÁ Veronika ŠANDEROVÁ Hana NOVÁČEK Jiří ŽÍDEK Lukáš KRÁSNÝ Libor SKLENÁŘ Vladimír

Conference abstract

Year: 2009, type: Conference abstract